JRC GMO-Amplicons | EURL GMFF

Record ID:	PP159197.1
Database:	nt	Go to original record
Description:	Cenchrus polystachios chloroplast, complete genome.
Taxon:	Cenchrus polystachios
Original size:	138171 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47971	48583	+	613 bp

Amplicon:

613 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tgataacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaaacaagt ggttctaaaa
151   ctggaaccca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg aagtaattac gttgtgttgg taggggaact ccctcgaaat
251   actagaaaga agggctttat acatttaata cacacgtata gatactgaca
301   tagtaaacga ttaatcacag aacccctatc ataatatagg ttctttattt
351   tatatttttt tctagaatga aattaggaat gattatgaaa tagaaaattc
401   tgaatttttt tagaattatt gtgaatccat tccaatcgaa tattgagtaa
451   tcaaatcctt caattcattg ttttgagatc aaaagtggat taatcgaacg
501   aggataaaga gagagtccca ttctacatgt caatactgac aacaatgaaa
551   tttctagtaa aaggaaaatc cgtcgacttt ataagtcgtg agggttcaag
601   tccctctatc ccc

View other 11 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.76e-08
primerB	100.0	20 bp	0	0	613	594	2.76e-08

Match details