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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)
Last update: 11/04/2025


GMOMETHODS

GMOMETHODS provides information on EU reference methods for GMO Analysis that have been validated according to the principles and requirements of international standards. Data is retrieved from peer-reviewed journals and final reports of collaborative studies.

Disclaimer

This EU Database of Reference Methods for GMO Analysis has been developed by the Joint Research Centre as European Union Reference Laboratory for GM Food and Feed (EU-RL GMFF), in collaboration with the European Network of GMO Laboratories (ENGL).

It aims at providing a list of reference methods for GMO analysis that have been:

  • validated in a collaborative trial, according to the principles and requirements of ISO 5725 and/or IUPAC protocol

or

  • verified by the EURL GMFF in the context of compliance with a EU legislative act.

All efforts have been made to ensure that the information provided is accurate and correct but no responsibility can be taken for the data and information reported in the GMOMETHODS database and the related PDF Summary.

The JRC does not own any Intellectual Property on the data and information reported in the method validation publication. Such data and information are owned by the method developers. Some legal provisions on confidentiality and data protection are provided in article 30 and article 31 respectively of Regulation (EC) No 1829/2003.

The European Union disclaims all implied representations and warranties, including any warranty of merchantability and fitness for a particular purpose. The entire risk as to the use, interpretation and fitness for a particular concrete purpose is with the user. Under no circumstance the European Union, the European Commission and/or any natural person acting on behalf of these entities shall be liable for any incidental, consequential, direct or indirect damages including but not limited to structural failure, loss of profits, or any other financial loss arising from the use of, or inability to use the data or any other matter relating to the service.

Last update (27/01/2025)
ID Description
QL-EVE-KP-001 Event specific method icon Qualitative PCR method for detection of Komagataella phaffii event MxY0541 (EURL GMFF, 2024)
QT-EVE-ZM-040 Event specific method icon Quantitative PCR method for detection of maize event DAS1131 (EURL GMFF, 2024)

Number of methods found: 222

Legend: Event specific method icon Event specific Element specific method icon Element specific Construct specific method icon Construct specific
Species specific method icon Species specific Plant specific method icon Plant specific

ID Sort by ID Event name Sort by event Species Sort by species Genetic target Sort by genetic target Description Sort by description
QL-CON-00-001 Construct specific method icon 40-3-2 (MON-04032-6) Soybean CaMV P-35S / CTP Qualitative PCR method for detection of the junction between the CaMV 35S promoter and the chloroplast transit peptide (CTP) sequence (ISO 21569:2005)
QL-CON-00-002 Construct specific method icon Tomato Nema 282F Tomato T-nos / PG Qualitative PCR method for detection of the junction between the polygalacturonase gene (PG) and the nopaline synthase (nos) terminator (ISO 21569:2005)
QL-CON-00-003 Construct specific method icon Bt11 (SYN-BT011-1) Maize IVS 2 ADH1 / pat Qualitative PCR method for detection of the junction between the intron 2 from the maize ADH1 gene and the pat gene (ISO 21569:2005)
QL-CON-00-004 Construct specific method icon Bt176 (176) (SYN-EV176-9) Maize P-CDPK / Cry1A(b) Qualitative PCR method for detection of the junction between the CDPK promoter from maize and the synthetic Cry1A(b) gene (ISO 21569:2005)
QL-CON-00-005 Construct specific method icon T25 (ACS-ZM003-2) Maize pat / CaMV T-35S Qualitative PCR method for detection of the junction between the pat gene and the CaMV 35S terminator (ISO 21569:2005)
QL-CON-00-006 Construct specific method icon 40-3-2 (MON-04032-6) Soybean CaMV P-35S / CTP Qualitative PCR method for detection of the junction between the CaMV 35S promoter and the chloroplast transit peptide (CTP) sequence (EU-Project SMT4-CT96-2072:1998)
QL-CON-00-007 Construct specific method icon Bt63 (TT51-1) Rice Cry1Ab/Ac / T-nos Qualitative PCR method for detection of the junction between Cry1Ab/Ac and DNA spacer sequences (Grohmann and Maede, 2009)
QL-CON-00-008 Construct specific method icon multiple events multiple species CP4-epsps / CTP2 Qualitative PCR method for detection of the junction between the CTP2 and the CP4-epsps gene (Grohmann et al., 2009)

Related publications:

  • Bonfini, L., et al. "GMOMETHODS: The European Union Database of Reference Methods for GMO Analysis." Journal of AOAC International, vol. 95, no. 6, 1 Nov. 2012, pp. 1713-1719.
  • Bonfini, L., "In Silico Proposal of Screening Strategies for Detecting EU Authorised GMOs." European Commission, Ispra, JRC131782, 2023.