JRC GMO-Amplicons compiles putative GMO-related amplicon sequences by performing in silico PCR simulation with primers of EU reference methods on public nucleotide sequence databanks.
The simulations for detecting potential amplicons are made using "re-PCR" (Rotmistrovsky et al, 2004) while the EU reference methods are retrieved from the GMOMETHODS database, a JRC application containing validated PCR-based detection and identification methods for GMO analysis. Public nucleotide sequence databanks include patents and available whole plant genomes.
The application supports control laboratories in the design and assessment of GMO methods, providing inter-alia in silico prediction of primers specificity and GM targets coverage. It can also assist in the detection and identification of unauthorized GMOs by allowing retrieval and characterization of GMO-related sequences from public databases and patents documentation.
Query the database for specific amplicons features
Inspect target sequences from public nucleotide sequence databanks
For more information, see:
© European Union, 2015
The information above is provided "as is" without warranty of any kind, either express or implied, including, but not limited to, fitness for a particular purpose and absence of errors in the application. The entire risk as to the use, quality, and performance of the application is with the user. The EU will not be liable for any incidental, consequential, direct or indirect damages including but not limited to the loss of data, loss of profits, or any other financial loss arising from the use or inability to use the application.