JRC GMO-Amplicons | EURL GMFF

Record ID:	KY430683.1
Database:	nt	Go to original record
Description:	Citrullus lanatus subsp. vulgaris cultivar PI482326 plastid, complete genome.
Taxon:	none
Original size:	156891 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49562	50147	+	586 bp

Amplicon:

586 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctttttccga aaacaaaaaa agggtaggtg
151   cagagactca atggaagctg ttctaacaaa tggagttgac tacgttgcgt
201   tagtaaagga atgaatcctt ccatcgaaac tccagaaaag atgaaagata
251   aacgtattac gtactgaaat actatattga ttaatgacaa ctcgaatctc
301   tatttttttt tatttatata taaaaaatga tatataaaaa tgaacgaatt
351   gttatgaatc gattccaata tctccaagtt gaaaaaagaa tcgaatattc
401   attgatcaaa tcatttactc catcatacat agtctgatag atcttttgaa
451   gaacttatta atcagatgcg aatagaataa agatagagtc ccattctaca
501   tgtcaatacc gacaaaaatg aaatttatag taagaggaaa atccgtcgac
551   tttaaaaatc gtgagggttc aagtccctct atcccc

View other 8 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	586	567	2.64e-08

Match details