JRC GMO-Amplicons | EURL GMFF

Record ID:	KX078552.1
Database:	nt	Go to original record
Description:	Rhodiola rosea genotype Sligo tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Rhodiola rosea
Original size:	963 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	604	+	604 bp

Amplicon:

604 bp

1     cgaaatcggt agacgctacg gacttaattg tattgagcct tggtatggaa
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ttcttttccg aaaccaaacc caaatacagg
151   ggttcaaaaa gcgcgaataa aaaaggatag gtgcagagac tcaacggaag
201   ttgttctaac aaatagagtt tactatgttg cattgacaaa ggaatctttt
251   cattgaaact ccagaaagga tgaaggataa atcttaataa acatatgtat
301   acctaataaa catatgtata cctaccgaaa tagtatatca aatgattaat
351   gacgactcaa atttttattt taaaatgaaa gaattgttag gaagcgattc
401   cgagttgaag aaagaatcga ctcttcattg ataaaataag tgtcactcca
451   cagtctgaga gatcttttga cgaactgaga ttaatcggac gagaataaag
501   atagagtccc attatacatg tcaatactga caacaaggaa attgatagta
551   aaaggaaaat ccgtcgactt tagaaatcat gagggttcaa gtccctctat
601   cccc

View other 6 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.72e-08
primerB	100.0	20 bp	0	0	604	585	2.72e-08

Match details