JRC GMO-Amplicons | EURL GMFF

Record ID:	KT597275.1
Database:	nt	Go to original record
Description:	Tetrastigma harmandii isolate 3283 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Tetrastigma harmandii
Original size:	1020 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	586	+	585 bp

Amplicon:

585 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct taatatggaa
51    acctactaag tgataacttt caaattcagg gaaaccctgg aataaaaaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccagaa aacgctaata
151   aaaaaaggat aggtgcagag actcaatgga agctgttcta acaaaaacaa
201   atggagttga ctatgttacg atgttaaacg aatccttcca tcaaaactcc
251   agaacccgaa aggatgaagg ataaacctat atacataggt actgaatgaa
301   atattatatc aaatgattaa tgatggctcg aatctctatt tttttatatg
351   aaaaatgaaa gaattgttgt aaatcgattc caagttgaag aaagaaaaga
401   atcgaatatt cattgatcaa atcattcact ccatagtccg atagatcatt
451   tgaagaactg attaatcgga cgagaataaa gagagagtcc cattctacat
501   gtcaatatcg acagcaatga aatttatagt aagaggaaaa tccgtcgact
551   ttaaaaatcg tgagggttca agtccctcta tcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	585	566	2.63e-08

Match details