JRC GMO-Amplicons | EURL GMFF

Record ID:	KT597270.1
Database:	nt	Go to original record
Description:	Tetrastigma cf. scariosum PBP-2017 isolate 3137 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Tetrastigma cf. scariosum PBP-2017
Original size:	966 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	553	+	552 bp

Amplicon:

552 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct taatatggaa
51    acctactaag tgataacttt caaattcagg gaaaccctgg aataaaaaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccagaa aacgctaata
151   aaaaaggata ggtgcagaga ctcaatggaa gctgttctaa caaaaatgga
201   gttgactatg ttacgatgtt aaacgaatcc ttccatcaaa actctagaac
251   cagaaaggat gaaggataaa cctatataca taggtactga aatattatat
301   caaatgatta atgatggctc gaatctctat tttttatatg aaaaatgaaa
351   gaattgttgt aaatcgattc caatattcat tgatcaaatc attcactcca
401   tagtccgata gatcatttga agaacggatt aatcggacga gaataaagag
451   agagtcccat tctacatgtc aatatcgaca gcaatgaaat ttatagtaag
501   aggaaaatcc gtcgacttta aaaatcgtga gggttcaagt ccctctatcc
551   cc

View other 3 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.48e-08
primerB	100.0	20 bp	0	0	552	533	2.48e-08

Match details