JRC GMO-Amplicons | EURL GMFF

Record ID:	KT597269.1
Database:	nt	Go to original record
Description:	Tetrastigma cf. ellipticum PBP-2017 isolate 3131 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Tetrastigma cf. ellipticum PBP-2017
Original size:	996 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	580	+	579 bp

Amplicon:

579 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct taatatggaa
51    acctactaag tgataacttt caaattcagg gaaaccctgg aataaaaaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccggaa aacgctaata
151   aaaaagaaag gataggtgca gagactcaat ggaagctgtt ctaacaaaaa
201   caaatggagt tgactatgtt acttaaatga atccttccat caaaactcca
251   gaaccagaaa ggatgaagga taaacctata tacataggta ctggtaatga
301   aatattatat caaatgatta atgatggctc gaatctctat ttttttatat
351   gaaaaatgaa agaattgttg taaatcgatt ccaaaaagaa aagaatcgaa
401   tattcattga tcaaatcatt cactccatag tccgatagat catttgaaga
451   acggattaat cggacgagaa taaagagaga gtcccattct acatgtcaat
501   atcgacagca atgaaattta tagtaagagg aaaatccgtc gactttaaaa
551   atcgtgaggg ttcaagtccc tctatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.61e-08
primerB	100.0	20 bp	0	0	579	560	2.61e-08

Match details