Match details

Record ID: ON641270.1
Database: nt Go to original record
Description: Lavandula angustifolia isolate BPTPS104 voucher LISE:96426 chloroplast, complete genome.
Taxon: Lavandula angustifolia
Original size: 151434 bp

Method: QL-PLN-00-007 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 46592 47154 + 563 bp

Amplicon: 563 bp
1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaactc aaaacaaggg
151   ttcaaaaaac gaaaaaaaaa aaaggatagg tgcagagact caatggaagc
201   tgttctaaca aatggagttg actgcgctga tagaggaatc tttccacgga
251   aactttagaa aggataaacg catctattga atactatatc aaatggttaa
301   tgatggcccg aatctttttt tttttaatat gaaaatggaa aaattggtat
351   gaattgattc tacgttgaag aaaaaatcga atattcatca actcattcgc
401   tccatagtcc gatagatctt ttaaagaact gattaatcgg acgagaataa
451   agatagagtc ccattctaca tgctacatgt caataccggc aacaatgaaa
501   tttatagtaa gaggaaaatc cgtcgacttt aaaaatcgtg agggttcaag
551   tccctctatc ccc 

View other 1 match with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 2.53e-08
primerB 100.0 20 bp 0 0 563 544 2.53e-08


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.