JRC GMO-Amplicons | EURL GMFF

Record ID:	ON641265.1
Database:	nt	Go to original record
Description:	Mentha suaveolens isolate BPTPS057 voucher LISE:96384 chloroplast, complete genome.
Taxon:	Mentha suaveolens
Original size:	151824 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46697	47267	+	571 bp

Amplicon:

571 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt cccaaaacaa aggtttcaaa
151   aaacgaaaaa aaaggatagg tgcagagact caatggaagc tgttctaaca
201   aatggagttg actgcgtcgg tagaggaatc tttccatgga aactttattt
251   tataaaggat gaaagataaa cgcacctatt gaatactata tcaaatgatt
301   aatgttggcc cgaatctatt tttttaatat gaaaatgaaa aaatggaaaa
351   atcggtgtga atttttttca cgttgaagaa aaaatagaat attcatcaac
401   tcattcactc cgtagtccga tagatctttt aaagaactta ttaatcggac
451   gagaataaag atagagtccc attctacatg ctacatatca ataccggcaa
501   caatgaaatt tatagtaaga ggaaaatccg tcgactttaa aaatcgtgag
551   ggttcaagtc cctctatccc c

View other 5 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.57e-08
primerB	100.0	20 bp	0	0	571	552	2.57e-08

Match details