JRC GMO-Amplicons | EURL GMFF

Record ID:	ON641263.1
Database:	nt	Go to original record
Description:	Lavandula stoechas subsp. luisieri isolate BPTPS052 voucher LISE:96380 chloroplast, complete genome.
Taxon:	Lavandula stoechas subsp. luisieri
Original size:	152039 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46968	47521	+	554 bp

Amplicon:

554 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcaaaa
151   aacgaaaaaa gggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttgac tgcgctgata gaggaatctt tccatggaga ctttagaaag
251   gatgaaggat aaacgcatct attgaatact atatcaaatg gttaatgatg
301   gcccgaatct ttttttttaa tatgaaaatg gaaaattggt gtgaattgat
351   tctacgttga agaaaaatcg aatattcatc aactcattcg ctccatagtc
401   cgatagatct tttaaagaac tgattaatcg gacgagaata aagatagagt
451   cccgttctac atgctacatg tcaataccgg caacaatgaa atttatagta
501   agaggaaaat ccgtcgactt taaaaatcgt gagggttcaa gtccctctat
551   cccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.49e-08
primerB	100.0	20 bp	0	0	554	535	2.49e-08

Match details