JRC GMO-Amplicons | EURL GMFF

Record ID:	ON641260.1
Database:	nt	Go to original record
Description:	Lavandula stoechas subsp. luisieri isolate BPTPS032 voucher LISE:96363 chloroplast, complete genome.
Taxon:	Lavandula stoechas subsp. luisieri
Original size:	152065 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46968	47522	+	555 bp

Amplicon:

555 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcaaaa
151   aacgaaaaaa agggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttga ctgcgctgat agaggaatct ttccatggag actttagaaa
251   ggatgaagga taaacgcatc tattgaatac tatatcaaat ggttaatgat
301   ggcccgaatc ttttttttta atatgaaaat ggaaaattgg tgtgaattga
351   ttctacgttg aagaaaaatc gaatattcat caactcattc gctccatagt
401   ccgatagatc ttttaaagaa ctgattaatc ggacgagaat aaagatagag
451   tcccgttcta catgctacat gtcaataccg gcaacaatga aatttatagt
501   aagaggaaaa tccgtcgact ttaaaaatcg tgagggttca agtccctcta
551   tcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.50e-08
primerB	100.0	20 bp	0	0	555	536	2.50e-08

Match details