JRC GMO-Amplicons | EURL GMFF

Record ID:	KX752086.1
Database:	nt	Go to original record
Description:	Alloteropsis semialata isolate Asem2 chloroplast, complete genome.
Taxon:	Alloteropsis semialata
Original size:	139660 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48400	49009	+	610 bp

Amplicon:

610 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaaataagt ggttgtcaaa
151   ctaaaaccca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg aagtaattac gttgtgttgg tagtgtaact ccctggaaat
251   tagagaaaga agggcacatc taatacacac gtatagatac tgacatagca
301   aacgattaat cacggaaccc atatcctaat ataggttctt tttttttatt
351   ttttagaatg aaattaggaa tgattatgaa atagaaaatt ctgaattttt
401   ttagaattat tgtgaatcca ttccaatcga atattgagta atcaaatcct
451   tcaattcatt gttttcgaga tctttaaaaa agtggattaa tcgaacgagg
501   ataaagagag agtcccattc tacatgtcaa tactgacaac aatgaaattt
551   ctagtaaaag gaaaatccgt cgactttata agtcgtgagg gttcaagtcc
601   ctctatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.75e-08
primerB	100.0	20 bp	0	0	610	591	2.75e-08

Match details