JRC GMO-Amplicons | EURL GMFF

Record ID:	OP748223.1
Database:	nt	Go to original record
Description:	Physalis ixocarpa chloroplast, complete genome.
Taxon:	Physalis ixocarpa
Original size:	156871 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49172	49743	+	572 bp

Amplicon:

572 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgatcacttt caaattcaga gaaaccctgg aattaacaaa
101   aatgggcaat cctgagccaa atcctgtttt ctgaaaacaa acaaaggttc
151   agaaaaaaag gataggtgca gagactcaat ggaagctatt ctaacaaatg
201   gagttaaatg cgttggtaga ggaatcttta catcgaaact tcagaaagaa
251   aaagaatgaa gtgaaggata aacgtatata catacgtatt gaatactata
301   tcaaatgatt aatgacgacc cgaatccgta ttttttctat aaaaaaatag
351   aagaattggt gtgaatcgat tctacattga agaaagaatc gaatattcat
401   tgatcaaatc attcactcca tagtctgata gatcttttga agaactgatt
451   aatcggacga gaataaagat agagtcccgt tctacatgtc aatgccggca
501   acaatgaaat ttatagtaag aggaaaatcc gtcgacttta aaaatcgtga
551   gggttcaagt ccctctatcc cc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	572	553	2.58e-08

Match details