JRC GMO-Amplicons | EURL GMFF

Record ID:	OR743512.1
Database:	nt	Go to original record
Description:	Eremophila cuneifolia isolate BMR07484 chloroplast, complete genome.
Taxon:	Eremophila cuneifolia
Original size:	151798 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48245	48810	+	566 bp

Amplicon:

566 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   agcgaaaaaa aaggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttga ctgcgttggt agaggaatct ttccatcgaa acttcagaaa
251   gaaaggatga aggataaagg tatatattga atactatatc aaatgattaa
301   tgatggccct aatctgtatc tgtatttttt tatatgaaaa atggaagaat
351   tggtatgaag tgattccaca ttgaagaaag aatcaaatat tccttcatca
401   aatcattcac tccacagtct gatagttctt ttaaagaact gattaatcgg
451   acaagaataa agatagagtc ccattctaca tgtcaatatt ggcaacaatg
501   caatttatag taagaggaaa atccgtcgac ttttaaagtc gtgagggttc
551   aagtccctct atcccc

View other 2 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	566	547	2.55e-08

Match details