JRC GMO-Amplicons | EURL GMFF

Record ID:	KX373096.1
Database:	nt	Go to original record
Description:	Denisophytum madagascariense voucher Lewis et al. 2158 (K) tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Denisophytum madagascariense
Original size:	1077 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	604	+	604 bp

Amplicon:

604 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctatcaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaaga agagttcaga
151   aagggaaagg gagaataaaa aaaggatagg tgcagagact caacggaagc
201   tgttctaaca aatggagttg acgacatttc ctttcgttcg taaaggaatt
251   cttccatcga aactccagaa aagaaaggat caaggatgaa catatatata
301   cgtatacgta ctgaaatact atttcaattg attagaccag acaaacccca
351   aatctctatt ttttaatatt tatatgacaa atgaaagatg tgaatagatt
401   ccaagttgaa gaaagaatcg aatatttatt gatcaaatca ttcactccat
451   catagtctga tagatctttt gaagaactga ttaatcggac gagaataaag
501   atagagtccc attctacatg tcaataacga caacaatgaa atttatagta
551   agaggaaaat ccgtcgactt tagaaatcgt gagggttcaa gtccctctat
601   cccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.72e-08
primerB	100.0	20 bp	0	0	604	585	2.72e-08

Match details