JRC GMO-Amplicons | EURL GMFF

Record ID:	OY787633.1
Database:	nt	Go to original record
Description:	Cruciata laevipes genome assembly, chromosome: 2
Taxon:	Cruciata laevipes
Original size:	31028921 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	24624215	24624834	+	620 bp

Amplicon:

620 bp

1     cgaaatcggt agacgctacg gacttaattg gattgggcct tggtatggaa
51    acctgctgag tgagagcttt caaattcaga gaaaccctgg aattaataaa
101   aaggggtaat cctgagccaa atcctatttt tcgaaaacaa aggtttagaa
151   agtgaaaaag ggataggtgc agagactcaa tggaagctgt tctaacaaat
201   ggagttcgct gtgttagtcg ataattcttt ccatcaaaaa ctcagaaaat
251   acaactacta agtattaagt gaaggataaa cgtatatact atccatacgt
301   agtgagtagt atattttata ttaaattttt aaaattatta aatgagtaat
351   gacagcccca atgaatctat actttttcat agaaaaaatt atagaattgt
401   aattgttgtt aaaatattcc acgtttaaga aagaatctaa tattcattta
451   tcaaatgatt cactccacag tccgatagat ctttttacga acttattaat
501   cggacgagaa taaagataga gtcccgttct acatgtcaat gccggcaaca
551   atgaaattta tagttaagag gaaaatccgt cgacttttaa aatcgtgagg
601   gttcaagtcc ctctatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.79e-08
primerB	100.0	20 bp	0	0	620	601	2.79e-08

Match details