JRC GMO-Amplicons | EURL GMFF

Record ID:	OR727014.1
Database:	nt	Go to original record
Description:	Eremophila naaykensii isolate BMR06434 chloroplast, complete genome.
Taxon:	Eremophila naaykensii
Original size:	151741 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48228	48795	+	568 bp

Amplicon:

568 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   agcgaaaaaa aaaaggatag gtgcagagac tcaatggaag ctgttctaac
201   aaatggagtt gactgcgttg gtagaggaat ctttccatcg aaacttcaga
251   aagaaaggat gaaggataaa ggtatatatt gaatactata tcaaatgatt
301   aatgatggcc ctaatctgta tctgtatttt tttatatgaa aaatggaaga
351   attggtatga agtgattcca cattgaagaa agaatcaaat attccttcat
401   caaatcattc actccacagt ctgatagttc ttttaaagaa ctgattaatc
451   ggacaagaat aaagatagag tcccattcta catgtcaata ttggcaacaa
501   tgcaatttat agtaagagga aaatccgtcg acttttaaag tcgtgagggt
551   tcaagtccct ctatcccc

View other 4 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	568	549	2.56e-08

Match details