JRC GMO-Amplicons | EURL GMFF

Record ID:	OR727010.1
Database:	nt	Go to original record
Description:	Eremophila magnifica subsp. magnifica isolate BMR06317 chloroplast, complete genome.
Taxon:	Eremophila magnifica subsp. magnifica
Original size:	151763 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48267	48824	+	558 bp

Amplicon:

558 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   agcgaaaaaa aaaaaggata ggtgcagaga ctcaatggaa gctgttctaa
201   caaatggagt tgactgcgtt ggtagaggaa tctttccatc gaaacttcag
251   aaaggataaa ggtatatatt gaatactata tcaaatgatt aatgatggcc
301   ctaatctgta tctgtatttt tttatatgaa aaatggaaga attggtatga
351   agtgattcca cattgaagaa agaatcaaat attccttcat caaatcattc
401   actccacagt ctgatagttc ttttaaagaa ctgattaatc ggacaagaat
451   aaagatagag tcccattcta catgtcaata ttggcaacaa tgcaatttat
501   agtaagagga aaatccgtcg acttttaaag tcgtgagggt tcaagtccct
551   ctatcccc

View other 8 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.51e-08
primerB	100.0	20 bp	0	0	558	539	2.51e-08

Match details