JRC GMO-Amplicons | EURL GMFF

Record ID:	OR699052.1
Database:	nt	Go to original record
Description:	Prostanthera althoferi subsp. althoferi voucher PBS YEEL 1 isolate BMR06606 chloroplast, complete genome.
Taxon:	Prostanthera althoferi subsp. althoferi
Original size:	152870 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48046	48614	+	569 bp

Amplicon:

569 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggtaat cctgagccaa atcccgtttt ctcaaaacaa aggtaaaggt
151   tcaaaaaacg aaaaaaagga taggtgcaga gactcaatgg aagctgttct
201   aacaaatgga gttgattgcc ctggtagagg catctttgca tggaaacttc
251   cgaaaggatg aaggataaat gtatctattg aatactatat cattaatgat
301   taatgatggt ccgaatctgt atttgtattt tttaatatga aaaatgtaag
351   aattgatgtg aattgattcc acattgaaga aaaaatcgaa tattcagtca
401   gcaaatgatt cactccatag tccaatagat cttttaaaga actgattaat
451   cggacgagaa taaagataga gtcccattct acatgtcaat accggcaaca
501   atgaaattta tagtaagagg aaaatccgtc gactttaaaa atcgtgaggg
551   ttcaagtccc tctatcccc

View other 4 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	569	550	2.56e-08

Match details