JRC GMO-Amplicons | EURL GMFF

Record ID:	OR730796.1
Database:	nt	Go to original record
Description:	Persicaria capitata voucher THL chloroplast, complete genome.
Taxon:	Persicaria capitata
Original size:	160070 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47580	48171	+	592 bp

Amplicon:

592 bp

1     cgaaatcggt agacgctacg gacttaattg gtttgagcct tagtatggaa
51    acttactaag tgataacttt caaactcaga gaaaccctgg aattaaaaaa
101   atgggcaatc ctgagcccac tcctgctttc caaaaggaaa gaataaaaaa
151   ggataggtgc agagactcaa tggaagctgt tctaacaaat ggagttgact
201   gtcttgcgtt gtgatatgtt ataagaattc ttcaatctaa actccaaaaa
251   ggatgaagaa gaaacctata ggcatacgta cttaaatact atggtataat
301   aaaaaaatat aaaatgaaaa aatattgata acgacccgaa tcttcgtttt
351   atgaatatga aaaaatggaa gaatttttgt gaatcaattc caagttgaag
401   aaagaatcga atattcgttt attaaattaa agcatttact ccccttgata
451   gatcttttga agaaccgatc catcggatga gaatgaagat agagtcccat
501   tctacgtgtc aaccccgaca acaacgaaat ttatagtaag aggaaaatcc
551   gtcgacttta taaatcgtga gggttcaagt ccctctatcc cc

View other 2 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.67e-08
primerB	100.0	20 bp	0	0	592	573	2.67e-08

Match details