JRC GMO-Amplicons | EURL GMFF

Record ID:	OR597584.1
Database:	nt	Go to original record
Description:	Cornus hongkongensis isolate organ chloroplast, complete genome.
Taxon:	Cornus hongkongensis
Original size:	156989 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48608	49201	+	594 bp

Amplicon:

594 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ccgaaaacaa acaaaaacaa
151   aggttcggaa aacgaaaata aaaaaagggg ataggtgcag agactcaatg
201   gaagctgttc taacacgtgg agttgactaa gttgtgttgt tagaagaatc
251   ttttttcatc gaaacttcag aaaggatgaa ggagaaaact atatacatac
301   gtatacgtac tgaaatatta tatcaaatga ttactgacga cacgaatctt
351   tattttttta tatgaaaaat caaagaattg ttgtgaatcg atttcaaatt
401   gaagaaagaa tccaatattc attgatcaaa tcattcactc cgtagtctga
451   tatatctttt gaagaactga ttaatcgaac gagaataaag atagagtccc
501   attctacatg tcaataccga caacaatgaa atttatagta agaggaaaat
551   ccgtcgattt tagaaatcgt gagggttcaa gtccctctat cccc

View other 2 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	594	575	2.68e-08

Match details