JRC GMO-Amplicons | EURL GMFF

Record ID:	OR582423.1
Database:	nt	Go to original record
Description:	Laguncularia racemosa chloroplast, complete genome.
Taxon:	Laguncularia racemosa
Original size:	160671 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	82285	82882	-	598 bp

Amplicon:

598 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgacaacttt caaattcaga gaaaccctgg aattcaaaaa
101   aatgggtaat cctgagccaa atcctatttt atgaaaacca acaaaacaac
151   aaagattcat aaagcaagaa taaaaaaagg ataggtgcag agactcaatg
201   gaagctgttc taacaaatgg agttgagcat gttgcgttgg tattggtaaa
251   ggaattcttt tatcgaaact ccagaaaggg aaaaaatgaa agataaacct
301   atatgcatac gtactgaata ctatctcaaa tgattaatga cgacgtgaat
351   ctcatctttt tctatatgaa aacgaaacga attgttgtga atcaattcga
401   agttgaaaaa agaatcgaat attcattgat taaatcattt cctccatggt
451   ctgagaaatc ttttgaagag cggattaatc agacgagaat aaagatagag
501   tcccattcta catgtcaata ctgacaacaa tgaaatttat agtaagagga
551   aaatccgtcg actttataaa tcgtgagggt tcaagtccct ctatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.69e-08
primerB	100.0	20 bp	0	0	598	579	2.69e-08

Match details