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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: OX637246.1
Database: nt Go to original record
Description: Oryza sativa genome assembly, chromosome: 1
Taxon: Oryza sativa
Original size: 51113606 bp

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Method: QT-TAX-OS-003 Go to GMOMETHODS record
Description:Quantitative PCR method for detection of rice sucrose-phosphate synthase gene (Jiang et al., 2009).
Target:rice sucrose-phosphate synthase

View other 14 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 2 47796206 47796286 - 81 bp
Amplicon: 81 bp 
1     ttgcgccctg acggatatct ttcagtttgt aaccaccgga tgacgcacgg
51    acggctcgga tcatcccgaa aagatcaacc g

View other 12 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerB 100.0 20 bp 0 0 81 62 4.00e-09
probe 100.0 19 bp 0 0 60 42 1.34e-08

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.