JRC GMO-Amplicons | EURL GMFF

Record ID:	OR438637.1
Database:	nt	Go to original record
Description:	Lilium leichtlinii var. maximowiczii chloroplast, complete genome.
Taxon:	Lilium leichtlinii var. maximowiczii
Original size:	152625 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	45131	45738	+	608 bp

Amplicon:

608 bp

1     cgaaatcggt agacgctacg gacttgatta aattgagcct tggtatggaa
51    acctgctaag tgataacttc caaattcaga gaaaccccgg aattaaaaat
101   gggcaatcct gagccaaatc tttattttga gaaaaagggt ttaatttata
151   gtttttttaa tataaaacta caataaaaaa agataggtgc agagactcaa
201   tggaagctgt tctaacgaat gaagttgatt acgttgcgct ggtagccgga
251   attcttctat caaaattaca gagaggatgc ccgccctata tacgtatata
301   tacatactga ccatagtaaa cgattgatta atcgcggccc gaatccatta
351   taatatatat atatgaaaaa tttaagagtt attgtaaatc tattccatat
401   tccaatcaaa gtttaaggaa gaatcgaata ttcagtgatc aaattattca
451   ttccagagtc tgtatagatc tttttaaaaa ctgattattc ggacgagaat
501   aaagagagag tcccattcta cgtgtcaata ccgacaacaa tgaaatttat
551   agtaaaagga aaatccgtcg actttataag tcgtgagggt tcaagtccct
601   ctatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.74e-08
primerB	100.0	20 bp	0	0	608	589	2.74e-08

Match details