JRC GMO-Amplicons | EURL GMFF

Record ID:	AH003085.2
Database:	nt	Go to original record
Description:	Nicotiana tabacum tRNA-Ser (Ser-tRNA), tRNA-Thr (Thr-tRNA), tRNA-Leu (Leu-tRNA), and tRNA-Phe (Phe-tRNA) genes, complete sequence; chloroplast.
Taxon:	Nicotiana tabacum
Original size:	2100 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	927	1503	+	577 bp

Amplicon:

577 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgatcacttt caaattcaga gaaaccctgg aattaacaaa
101   aatgggcaat cctgagccaa atcctgtttt ccgaaaacaa acaaaggttc
151   agaaaaaaag gataggtgca gagactcaat ggaagctatt ctaacaaatg
201   gagttaaatg cgttggtaga ggaatcttta catcgaaact tcagaaagaa
251   aaagaatgaa gtgaaggata aacgtatata catacgtatt gaatactata
301   tcaaaatcaa atgattaatg atgacccgaa tctgtatttt ttctataaaa
351   aatagaagaa ttggtgtgaa tcgattctac attgaagaaa gaatcgaata
401   ttcattgatc aaaccattca ctccatagtc tgatagatct tttgaagaac
451   tgattaatcg gacgagaata aagatagagt cccgttctac atgtcaatac
501   cggcaacaat gaaatttatc gtaagaggaa aatccgtcga ctttaaaaat
551   cgtgagggtt caagtccctc tatcccc

View other 13 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.60e-08
primerB	100.0	20 bp	0	0	577	558	2.60e-08

Match details