JRC GMO-Amplicons | EURL GMFF

Record ID:	OR415857.1
Database:	nt	Go to original record
Description:	Berberis amurensis isolate Ber_50_Mt_Neko chloroplast, complete genome.
Taxon:	Berberis amurensis
Original size:	166699 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48150	48711	+	562 bp

Amplicon:

562 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttcat aaaaaggtgg ttcagaaaga
151   aaaaaaaaag gataggtgca gagactcaat ggaagctgtt ctaacgaata
201   gggttgactg cgttggtcga ggaatctttc tatcgaaact ccggaaagga
251   tgaacctata tacgtacgta tttgtactga aatagcaaaa attaatgaga
301   tccgaatcca ttttttattt tatatgtata tgaaaaattt aaaatggatt
351   gtgaatcgat tccaaatgga aggaagaatc gaatattcgc cgatcaaatc
401   agtcactcta tggtctgata gttcttttga agaactaact tattggacga
451   gagtaaagat agagtcccgt tctacatgtc aataccgaca acaatgaaat
501   ttatagtaag aggaaaatcc gtcgacttta gaaatcgtga gggttcaagt
551   ccctctatcc cc

View other 6 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	562	543	2.53e-08

Match details