JRC GMO-Amplicons | EURL GMFF

Record ID:	OY730200.1
Database:	nt	Go to original record
Description:	Pseudognaphalium luteoalbum genome assembly, organelle: plastid:chloroplast
Taxon:	Pseudognaphalium luteoalbum
Original size:	153410 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49441	49961	+	521 bp

Amplicon:

521 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcacgtttt ccgaaaacaa acaaaggttc
151   agaaagcgaa aatcaaaagg ataggtgcag agactcgatg gaagctgttc
201   taacaaatgg agttgattgt cttacattgg tagagaaatc cttctataga
251   aacttcagaa aagatgaagg ataaacctgt atacataata gagatacaga
301   agaattgata tgaatcgatt ccatattgaa gaaagaatcg aatattcatt
351   gatcaaacca ttcactccat aatctgatag atcttttgaa gaactgatta
401   atcggacgag aataaagata gagtcccgtc ctacatgtca ataccggcaa
451   caatgaaatt tatagtaaga ggaaaatccg tcgatttcaa aaatcatgag
501   ggttcaagtc cctctatccc c

View other 17 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.34e-08
primerB	100.0	20 bp	0	0	521	502	2.34e-08

Match details