JRC GMO-Amplicons | EURL GMFF

Record ID:	OR260144.1
Database:	nt	Go to original record
Description:	Hemerocallis sp. isolate 87 cultivar Purple Water chloroplast, complete genome.
Taxon:	Hemerocallis hybrid cultivar
Original size:	156053 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47988	48571	+	584 bp

Amplicon:

584 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tagtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aactaaaaat
101   gggcaatcct gagccaaatc tttttttttc aaaaaaggtt tttttttgaa
151   actcgaataa aaaaagatag gtgcagagac tcaatggaag ctgttctaac
201   gaatggagtt gactacgttg tgttggtagc cggaatcctt ctatcgaaat
251   taaaaaaagg atgactatat atctaataaa tacgtataca tactgacata
301   tcaaacgatt aatcatgacc cgaattagta ttatatatat gcaaaattca
351   gagttattgt ggatctattc caatcgaagt tgaaggaaga acagaatatt
401   taatgatcaa atcattcatt ccagggtttg ataggccttt actttttttt
451   gacaaactga ttaatcggac gagaataaag agagagtccc attctacatg
501   tcaataccga caacaatgaa atttatagta agaggaaaat ccgtcgactt
551   taaaaatcgt gagggttcaa gtccctctat cccc

View other 17 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	584	565	2.63e-08

Match details