JRC GMO-Amplicons | EURL GMFF

Record ID:	OR260119.1
Database:	nt	Go to original record
Description:	Hemerocallis citrina isolate 30 cultivar Huohuanghua chloroplast, complete genome.
Taxon:	Hemerocallis citrina
Original size:	155877 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47942	48527	+	586 bp

Amplicon:

586 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tagtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aactaaaaat
101   gggcaatcct gagccaaatc tttttttttt caaaaaaggt tttttttttg
151   aaactcgaat aaaaaaagat aggtgcagag actcaatgga agctgttcta
201   acgaatggag ttgactacgt tgtgttggta gccggaatcc ttctatcgaa
251   attcaaaaaa ggatgactat atatctaata catacgtata catactgaca
301   tatcaaacga ttaatcatga cccgaattag tattatatat atgaaaaatt
351   cagagttatt gtggatctat tccaatcgaa gttgaaggaa gaacagaata
401   tttaatgatc aaatcattca ttccagggtt tgataggcct ttactttttt
451   ttgacaaact tattaatcgg acgagaataa agagagagtc ccattctaca
501   tgtcaatacc gacaacaatg aaatttatag taagaggaaa atccgtcgac
551   tttaaaaatc gtgagggttc aagtccctct atcccc

View other 7 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	586	567	2.64e-08

Match details