JRC GMO-Amplicons | EURL GMFF

Record ID:	ON229582.1
Database:	nt	Go to original record
Description:	Persicaria longiseta var. rotundata voucher SD658 chloroplast, complete genome.
Taxon:	Persicaria longiseta var. rotundata
Original size:	159401 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47693	48292	+	600 bp

Amplicon:

600 bp

1     cgaaatcggt agacgctacg gacttaattg gtttgagcct tagtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccctgg aattaaaaaa
101   aatgggcaat cctgagccaa ctcctgcttt caaaaaggaa agaaaaaggg
151   gataggtgca gagactcaat ggaagatgtt ctaacaaatg gaattgactg
201   gcttgcgttg taatatgtta taagaattct tcgatctaaa cgccaaaaag
251   gatgaagaag aaacctgcat aggtacttaa atagaaagac tatactatga
301   tataataata ataaaaaata caaaaatatt gatgacgaac cgaaattttc
351   ttttatgaat atgaaaaaaa tggaagaatt tttttgaatc gattctaagt
401   tgaagaaaga atcgaatatt cgtttattaa attaaagcat ttactccaca
451   gtttggtata tcttttgaag aaccgatcca tcggatgaga atgaagatag
501   agtcccattc tacgtgtcaa tcccgacaac aatgaaattt atagtaagag
551   gaaaatccgt cgactttata aatcgtgagg gttcaagtcc ctctatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.70e-08
primerB	100.0	20 bp	0	0	600	581	2.70e-08

Match details