JRC GMO-Amplicons | EURL GMFF

Record ID:	OP136019.1
Database:	nt	Go to original record
Description:	Dianthus chinensis chloroplast, complete genome.
Taxon:	Dianthus chinensis
Original size:	149641 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46671	47286	+	616 bp

Amplicon:

616 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tagtatggaa
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aataaaaaaa
101   agggtaatcc tgagccaact ccttttgttt ccttttttca aaagaaaaaa
151   aggataggtg cagagactca aaggaagctg ttctaacaaa ttgggttgac
201   tgtgctgtgt tgttataaga cttcttccgt ctaaattccc ataaaaaggg
251   gggtaaaatc gacggagtga aatgattaat gacaacccga atctgttctg
301   tattttttta tatatattat atatataaat ccatacttta ttctatattt
351   agagtagaga tctagaagat taaatgaaaa aatgcaagaa ttcttgtgaa
401   tccattcaaa gttgaaagca gaatcaatca agatttattc attaaaatat
451   tcacactaac tccatagtct gatagatctt gtgaagaact tattaatcgg
501   atgagaataa agatagagtc ccgtactaca tgtcaatact gacaacaatg
551   aaatttatag taagaggaaa atccgtcgac tttaaaaatc atgagggttc
601   aagtccctct atcccc

View other 3 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	616	597	2.78e-08

Match details