JRC GMO-Amplicons | EURL GMFF

Record ID:	OY292393.1
Database:	nt	Go to original record
Description:	Verbena officinalis genome assembly, organelle: plastid:chloroplast
Taxon:	Verbena officinalis
Original size:	153455 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49246	49798	+	553 bp

Amplicon:

553 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   aacgacaaag agggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttga ctgcgtgggt agaggaatct ttccatcgaa acttccgaaa
251   ggatgaagga taaacgtatc tattgaatac tctatcaaat gattaatgag
301   gtgtatctgt attttttgat atgaaaaatg gaagaattgg tgtgaattga
351   ttccacattg aagaaagaat cgaatattcc ttgatcaaat cattcactcc
401   atagtccgat aaatctttga aagaactgat taatcggacg agaataaaga
451   tagagtccca ttctacatgt caataccggc aagaatgaaa tttattgtaa
501   gaggaaaatc cgtcgacttt aaaaatcgtg agggttcaag tccctctatc
551   ccc

View other 9 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.49e-08
primerB	100.0	20 bp	0	0	553	534	2.49e-08

Match details