JRC GMO-Amplicons | EURL GMFF

Record ID:	OY282639.1
Database:	nt	Go to original record
Description:	Phragmites australis genome assembly, organelle: plastid:chloroplast
Taxon:	Phragmites australis
Original size:	137672 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48848	49464	+	617 bp

Amplicon:

617 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaaacaggt ggttctcaaa
151   ctagaaccca aagggaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg aggtaattac gttgtgttgg tagtgaaact ccctctaaat
251   tagagaaaga agggctttat acatctaata cacacctata gatactgaca
301   tagcaaatga ttaatcacag aacccatatc ataatatagg ttctttattt
351   atttattttt tagaatgaaa ttaggaatga ttatgaaata gaaaattctg
401   aattttttta gaattgttgt gaatccattc caatcgaata ttgagtaatc
451   aaatccttca attcattgtt tttgagatct tttaaaaagt ggattaatcg
501   gacgaggata aagagagagt cccattctac atgtcaatac tgacaacaat
551   gaaatttcta gtaaaaggaa aatccgtcga ctttataagt cgtgagggtt
601   caagtccctc tatcccc

View other 8 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	617	598	2.78e-08

Match details