JRC GMO-Amplicons | EURL GMFF

Record ID:	OQ743999.1
Database:	nt	Go to original record
Description:	Tetraena mongolica plastid, complete genome.
Taxon:	Tetraena mongolica
Original size:	106189 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47159	47695	+	537 bp

Amplicon:

537 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acatactaag tgatcacttt caaattcaga gaaaccctgg aattagaaat
101   gggcaatcct gagccaaatc ctgttttctg aaaaaaaaaa aataattcaa
151   tcaaaagaaa aagaatcaaa aaaggatagg tgcagagact caatggaagc
201   tgttctaaaa aatggagttg actgctgcat tatgttagtc aagtcaagga
251   atccttgcat aaaaactttc aaaaagatga aggataaact ttctttctat
301   aaaactctaa aaagaaaaat actttttcaa tcaattggaa gttgaataaa
351   gaatcaaata taatttgatc aaatcattta ctccaaagtc tgatatatct
401   ttttcaaaat ggattagtca gatgagaata aagatagagt cccattctat
451   atgtcagtat tgacaacaat gaaatttata gtgagaggaa aatccgtcga
501   ttttataaat cgtgagggtt caagtccctc tatcccc

View other 6 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.42e-08
primerB	100.0	20 bp	0	0	537	518	2.42e-08

Match details