JRC GMO-Amplicons | EURL GMFF

Record ID:	OX485189.1
Database:	nt	Go to original record
Description:	Oryza meyeriana genome assembly, organelle: plastid:chloroplast
Taxon:	Oryza meyeriana
Original size:	135917 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47788	48402	+	615 bp

Amplicon:

615 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   ggggcaatcc tgagccaaat ccgtgttttg agaaaacaag cggttctcga
151   actagaatcc aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gagttaatta cgttgtgttg gtagtggaat tcccttctaa
251   attagagaaa gaagggcttt cgaaacctaa tacacacgta tagatactgg
301   catagcaaac gattaatcac agaactcata tcataatata ggttctttaa
351   ttctttttta gaatgaaaat aggaatgatt atgaaataga aaattcataa
401   tttttttaga attgttgtga atccattcca atcgaatatt gagtaatcaa
451   atccttcaat tcatagtttt cgaaatcttt taaaaagtgg attaatcgga
501   cgaggataaa gagagagtcc cattctacat gtcaatactg acaacaatga
551   aatttctagt aaaaggaaaa tccgtcgact ttctaagtcg tgagggttca
601   agtccctcta tcccc

View other 2 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.77e-08
primerB	100.0	20 bp	0	0	615	596	2.77e-08

Match details