JRC GMO-Amplicons | EURL GMFF

Record ID:	KT124249.1
Database:	nt	Go to original record
Description:	Pinguicula bohemica isolate BO_SH_a trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Pinguicula bohemica
Original size:	1579 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	680	1236	+	557 bp

Amplicon:

557 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    cctactaagt gataactttc aaattcagag aaaccccgga attaataaaa
101   atgggcaatc ctgagccaaa tcctgttttc tcaaaagaaa ggttccgaaa
151   acgaaaaaaa aaaaaaggat aggtgcagag actcaatgga agctgttcta
201   acaaatgggg ttgaccgcga tggtagagga atctttccgc cgaaacttca
251   gagaggatca aggataaaca tatctattga atactatatc aaatgattac
301   tgatggcacg aatctctatt tttatatgaa aaatggaaga attggtgtga
351   attgattcca cattgaagaa aaatcgaata tttattcatc aaaccattca
401   ctccatagtc cgatatacct tttaacgaac tgatcaatcg gacaagaata
451   aagatagagt cccattctac atgtcaatac cggcaacaat gcaatttata
501   gtaagaggaa aatccgtcga ctttaaaaat cgtgagggtt caagtccctc
551   tatcccc

View other 3 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.51e-08
primerB	100.0	20 bp	0	0	557	538	2.51e-08

Match details