JRC GMO-Amplicons | EURL GMFF

Record ID:	MT650315.1
Database:	nt	Go to original record
Description:	Eremophila platycalyx voucher MELUD119248a plastid, complete genome.
Taxon:	Eremophila platycalyx
Original size:	151928 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48291	48857	+	567 bp

Amplicon:

567 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   agcgaaaaaa aaaggatagg tgcagagact caatggaagc tgttctaaca
201   aatggagttg actgcgttgg tagaggaatc tttccatcga aacttcagaa
251   agaaaggatg aaggataaag gtatatattg aatactatat caaatgatta
301   atgatggccc taatctgtat ctgtattttt ttatatgaaa aatggaagaa
351   ttggtatgaa gtgattccac attgaagaaa gaatcaaata ttccttcatc
401   aaatcattca ctccacagtc tgatagttct tttaaagaac tgattaatcg
451   gacaagaata aagatagagt cccattctac atgtcaatat tggcaacaat
501   gcaatttata gtaagaggaa aatccgtcga cttttaaagt cgtgagggtt
551   caagtccctc tatcccc

View other 10 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	567	548	2.55e-08

Match details