JRC GMO-Amplicons | EURL GMFF

Record ID:	OP262739.1
Database:	nt	Go to original record
Description:	Brandisia rosea var. flava isolate 1 voucher KUN:ChenZ-023 plastid, complete genome.
Taxon:	Brandisia rosea var. flava
Original size:	155112 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48212	48773	+	562 bp

Amplicon:

562 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaagaaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   aacgaaaaaa aaaaaaggat aggtgcagag actcaatgga agctgttcta
201   acaaataaaa tggagttgat tgcgctggta gaggaatgaa acttcagaaa
251   ggatgaagga taaacgtatc tattgaatac tatatcaaaa tggttgatta
301   atgatggccc gaatctgtat ctgtattttt gaatacgaaa aatggaagaa
351   ttggtgtgaa ttgattccat attgaagaaa gaatattcat tcatcaaatt
401   cttcactcca tagtccgata gatcttttaa agaactgatt aatcggacga
451   gaataaagat agagtcccat tctacatgtc aataccggca acaatgaaat
501   ttatagtaag aggaaaatcc gtcgacttta aaaatcgtga gggttcaagt
551   ccctctatcc cc

View other 3 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	562	543	2.53e-08

Match details