JRC GMO-Amplicons | EURL GMFF

Record ID:	AP014968.1
Database:	nt	Go to original record
Description:	Oryza sativa Japonica Group DNA, chromosome 12, cultivar: Nipponbare, complete sequence.
Taxon:	Oryza sativa Japonica Group
Original size:	27531856 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11283706	11284319	+	614 bp

Amplicon:

614 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggcaatttc caaattcaga gaaaccctgg aattaaaaaa
101   ggggcaatcc tgagccaatc catgttttga gaaaacaagt ggttctcgaa
151   ctagaaccca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg agttaattac gttgtgttgc tagcggaact cccttctaaa
251   ttagggaaag aagggctttc gaaatctaat acacacgtat agatactggc
301   atagcaaatg attaatcaca gaactcatat cataatatag gttctttaat
351   tcttttttaa aatgaaaata ggaatgatta tgaaatagaa aattcataat
401   ttttttagaa ttgttgtgaa tccattccaa tcgaatattg agtaatcaaa
451   tccttcaatt catagttttc gaaatctttt taaaagtgga ttaatcggac
501   gaggataaag agagagtccc attctacatg tcaatactga caacaatgaa
551   atttctagta aaaggaaaat ccgtcgactt tctaagtcgt gagggttcaa
601   gtccctctat cccc

View other 9 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.77e-08
primerB	100.0	20 bp	0	0	614	595	2.77e-08

Match details