JRC GMO-Amplicons | EURL GMFF

Record ID:	KF965267.1
Database:	nt	Go to original record
Description:	Tamilnadia uliginosa isolate BQ082 trnT-trnL intergenic spacer and tRNA-Leu (trnL) gene, partial sequence; chloroplast.
Taxon:	Tamilnadia uliginosa
Original size:	1740 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	802	1367	+	566 bp

Amplicon:

566 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcccgtttt ccgaaaccaa aggttcagaa
151   agtgaaaaag gataggtgca gagactcaac ggaagctgtt ctaacaaatg
201   gagttggctg cgttagtaga gaaatctttc catctaaaat tccgaaagga
251   taaagtgaag gataaacgta tatatacgta ttgaatacta tatcaaatga
301   ttaatgacga cccgactgaa tctgtatttt ttatatgaaa gcggaagaat
351   tggtgtgaat agattccaca ttgaagaaag aatcgaatat tcattgatca
401   aatgtttcac tccatagtct gatagatctt ttcaagaatt gattaatcgg
451   acgagaataa agatagagtc ccgttctaca tgtcaatgtc ggcaacaatg
501   aaatttatag taagaggaaa atccgtcgac tttaaaaatc gtgagggttc
551   aagtccctct atcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	566	547	2.55e-08

Match details