JRC GMO-Amplicons | EURL GMFF

Record ID:	KF965261.1
Database:	nt	Go to original record
Description:	Sericanthe andongensis isolate AL070 trnT-trnL intergenic spacer and tRNA-Leu (trnL) gene, partial sequence; chloroplast.
Taxon:	Sericanthe andongensis
Original size:	1771 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	809	1373	+	565 bp

Amplicon:

565 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcccgtttt ccgaaaccaa aggttcataa
151   agtgaaaaaa ggataggtgc agagactcaa cggaagctgt tctaacaaat
201   ggggttggct gcgttagtag agaaatcttc ccatctaaaa ttccgaaagg
251   ataaagtgaa ggataaacgt atatatgtat tgaatactat atcaaatgat
301   taatgacgac tcgactgaat ctgtattttt tatataaaaa cggaagaatt
351   ggtgtgaata gattccacat tgaagaaaga atcgaatatt cattgatcaa
401   atgattcact ccatagtctg atagatcttt tcaagaattg attaatcgga
451   cgagaataaa gatagagtcc cgttctacat gtcaatgtcg acaacaatga
501   aatttatagt aagaggaaaa tccgtcgact ttaaaaatcg tgagggttca
551   agtccctcta tcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	565	546	2.54e-08

Match details