JRC GMO-Amplicons | EURL GMFF

Record ID:	KF965159.1
Database:	nt	Go to original record
Description:	Aoranthe penduliflora isolate O059 trnT-trnL intergenic spacer and tRNA-Leu (trnL) gene, partial sequence; chloroplast.
Taxon:	Aoranthe penduliflora
Original size:	1668 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	764	1339	+	576 bp

Amplicon:

576 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcccgtttt ccgaaaccaa aggttcagaa
151   agtgaaaaaa ggataggtgc agagactcaa cggaagctgt tctaacaaat
201   ggagttggct gcgttagtag agaaatcttt ccatctaaaa ttccgaaagg
251   ataaagtgaa ggataaacgt atatatacgt attgaatact atatcaaatg
301   attaatgatg actcgactga atctgtattt tttatatgaa ttatatgaaa
351   acggaagaat tggtgtgaat agattccaca ttgaagaaag aatcgaatat
401   tcattgatca aatgattcac tccatagtct gatagatctt ttcaagaatt
451   gattaatcgg acgagaataa agatagagtc ccgttctaca tgtcaatgtc
501   ggcaacaatg aaatttatag taagaggaaa atccgtcgac tttaaaaatc
551   gtgagggttc aagtccctct atcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.59e-08
primerB	100.0	20 bp	0	0	576	557	2.59e-08

Match details