Match details
| Record ID: | LC057504.1 | |
|---|---|---|
| Database: | nt | Go to original record |
| Description: | Binary vector pMpGWB225 DNA, complete sequence. | |
| Taxon: | Binary vector pMpGWB225 | |
| Original size: | 13803 bp | |
View other 22 matches for this record
| Method: | QT-ELE-00-004 | Go to GMOMETHODS record |
|---|---|---|
| View in JRC GMO-Matrix | ||
| Description: | Quantitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter | |
| Target: | CaMV P-35S | |
View other 15625 matches for this method
Predicted amplification details:
| Mismatches | Gaps | From | To | Strand | Amplicon size |
|---|---|---|---|---|---|
| 0 | 0 | 960 | 1041 | + | 82 bp |
| Amplicon: | 82 bp |
|---|
1 gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat 51 cgtggaaaaa gaagacgttc caaccacgtc tt
View other 26489 matches with an identical amplicon
Primers and probe alignment:
| Primer/Probe | % Identity | Length | Mismatches | Gaps | From | To | Score |
|---|---|---|---|---|---|---|---|
| primerA | 100.0 | 18 bp | 0 | 0 | 1 | 18 | 4.54e-08 |
| primerB | 100.0 | 22 bp | 0 | 0 | 82 | 61 | 3.55e-10 |
| probe | 100.0 | 22 bp | 0 | 0 | 21 | 42 | 3.55e-10 |
Related publications:
- Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
- Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.