QT-ELE-00-004
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event 40-3-2 | MON-04032-6 (Soybean)
|
| Genetic target: | CaMV P-35S |
| Description: | Quantitative PCR method for detection of CaMV 35S promoter (Pauli et al., 2001) |
| Comments: | Element-specific and construct-specific methods may generate amplicons with different sequence or length depending on the GMO events where the element or construct is found. |
Method details
| Specificity: | Element specific |
| Analysis type: | Quantitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
GCCTCTGCCGACAGTGGTCCCAAAGATGGACCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTC TT Genbank ID: V00141 |
| Amplicon size: | 82 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | 35S-F / | GCCTCTGCCGACAGTGGT | 18 bp | CaMV P-35S |
| Primer reverse | 35S-R / | AAGACGTGGTTGGAACGTCTTC | 22 bp | CaMV P-35S |
| Probe | 35S-TMP / | FAM-CAAAGATGGACCCCCACCCACG-TAMRA | 22 bp |
