JRC GMO-Amplicons | EURL GMFF

Record ID:	OP133598.1
Database:	nt	Go to original record
Description:	Secamone elliptica subsp. siamica voucher C.F. Zhang 5992 chloroplast, complete genome.
Taxon:	Secamone elliptica subsp. siamica
Original size:	154770 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48212	48770	+	559 bp

Amplicon:

559 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtaaggaa
51    acctactaag tgatgacttt caaattcaga gaaaccccgg aattaagaaa
101   aagggcaatc ctgagccaaa tcctattttc cacaaacaaa ggttcagaaa
151   acgaaaacaa ggataggtgc agagactcga cggaagctgt tctaacaaat
201   ggagttggcc gcgttggtag agaaatcttt ccatcgaaaa ttcagaaagg
251   atgaaagata tacatacgta ttgaatacta tatcaaatga ttaatgccga
301   cccgaatgaa tctgtatttt tctataaaaa acggaagaat tggtgtgatt
351   cgattctttc ttcaatgtgg aatcgaatat tcattgatca aatgattcac
401   tccatagtct gtagatcctc ttttcaagaa ccggattaat cggacgagaa
451   taaagataga gtcccgttct acatgtcaat gctggcaaca atgaaattta
501   tagtaagagg aaaatccgtc gacttaaaaa atcgtgaggg ttcaagtccc
551   tctatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.52e-08
primerB	100.0	20 bp	0	0	559	540	2.52e-08

Match details