JRC GMO-Amplicons | EURL GMFF

Record ID:	ON872370.1
Database:	nt	Go to original record
Description:	Gynostemma burmanicum (nom. inval.) chloroplast, complete genome.
Taxon:	Gynostemma burmanicum (nom. inval.)
Original size:	157834 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49248	49857	+	610 bp

Amplicon:

610 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctttttccga aaacaaatca aataagggct
151   tcgaaagcga gaaaaaaaag ggtaggtgca gagactcaat ggaagctgtt
201   ctaacaaatg agttgactag gttgcgttag taaaggaatg aatccttcca
251   tcgaaactcc agaaaagatg aaagataaac gtatatacgt actgaaatac
301   tatttgatta atgacagccc gaatctctat tttttatagt tatctaaaaa
351   atgatatata tatataaaaa acgaattgtt attaatcgat tccaatatct
401   ccaggttgaa aaaagaatcg aatattcatt gatcaaatca tttactccat
451   catagtctga tagatctttt gaagaactga ttaatcagat gagaatagaa
501   taaagataga gtcccattct acatgtcaat accgacaaaa atgaaattta
551   tagtaagagg aaaatccgtc gacttaaaaa aatcgtgagg gttcaagtcc
601   ctctatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.75e-08
primerB	100.0	20 bp	0	0	610	591	2.75e-08

Match details