JRC GMO-Amplicons | EURL GMFF

Record ID:	OQ101230.1
Database:	nt	Go to original record
Description:	Amana wanzhensis isolate WMZ1455 chloroplast, complete genome.
Taxon:	Amana wanzhensis
Original size:	151264 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	45121	45683	+	563 bp

Amplicon:

563 bp

1     cgaaatcggt agacgctacg gacttgatta aattgagcct tggtatggaa
51    acctgctaag tgataacttc caaattcaga gaaaccccgg aattaaaaat
101   gggcaatcct gagccaaatc tttttttttc aaaaaaagga taggtgcaga
151   gactcaatgg aagctgttct aacaaatgga gttgattacg ttacatgcgc
201   tagtagccgg aatccttcta tcaaaattat tgaaaagata cccgccctat
251   atatgtagat atacatactc acatagtaaa cgattgatta atcgcagccc
301   aaatccatta tatatgaaaa atttaagaat cattgtgaat ctattccatt
351   cgatattcca atcgaagttg gaggaagaat cgaatattaa atgatcaaat
401   tattcattcc aaagtctgat agatcttttg aaaaaccgat tattcgaacg
451   agaataaaga gagagtccca ttctacgtgt caataccgac aacaatgaaa
501   tttatagtaa aaggaaaatc cgtcgacttt aaaagtcgtg agggttcaag
551   tccctctatc ccc

View other 2 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	563	544	2.53e-08

Match details