JRC GMO-Amplicons | EURL GMFF

Record ID:	OP837957.1
Database:	nt	Go to original record
Description:	Scutellaria baicalensis isolate SBW chloroplast, complete genome.
Taxon:	Scutellaria baicalensis
Original size:	151702 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47416	47980	+	565 bp

Amplicon:

565 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tgatgtggga
51    aacctactaa gtgataactt tcaaattcag agaacccccg gaattaataa
101   aaatgggtga tcctgatcca aatcctgttt tctgaaaacc tgaaaacaaa
151   ggctcaaaaa acgaaaaatg ggataggtgc agagactcaa tggaagctgt
201   tctaacaaat ggagttggct gcgttgttag aggaatcctt tccgcggaaa
251   cttcagaaag gatgaaggat aaacgtatct attgaatact atatcaaatg
301   attaatgatg gcccgaatcc gtatttttga atatgaaaaa acggaagaat
351   cggtgtgaat tgattccaca ttgaaaaaaa atcgaatatt cattcatcaa
401   atcattcact ccacggtcca ataggtcttt taaagaacta attaattgga
451   cgagaataaa gatagagtcc cattctacat gtcaataccg gcaacaatga
501   aatttatagt aagaggaaaa tccgtcgact ttaaaaatcg tgagggttca
551   agtccctcta tcccc

View other 8 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	565	546	2.54e-08

Match details