JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ427950.1
Database:	nt	Go to original record
Description:	Trichosanthes kirilowii isolate 16080 chloroplast, complete genome.
Taxon:	Trichosanthes kirilowii
Original size:	156980 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49792	50378	+	587 bp

Amplicon:

587 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctttttccga aaaaaaaagg gtaggtgcag
151   agactcaatg gaagctgttc taacaaatgg agttgactac gttgcgttag
201   taaaggaatg aatccttcta tcgaaactcc agaaaagatg aaagataaac
251   gtattacgta ctgaaatact atttgattaa tgacaacccg aatctccatt
301   tttttttttt ttttatttat atacaaaatg atatataaaa atgaacgaat
351   tgttctgaat cgattccaat atctccaagt tgaaaaaaga atcgaatatt
401   cattgatcaa atcatttact ccatcataca tagtctgata gatcttttga
451   agaactgatt aatcagatgc gaatagaata aagatagagt cccattctac
501   atgtcaatac cgacaaaaat gaaatttata gtaagaggaa aatccgtcga
551   ctttaaaaat cgtgagggtt caagtccctc tatcccc

View other 2 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	587	568	2.64e-08

Match details